Akadeum’s Human CD4+ T Cell Isolation Kit leverages flotation to deliver a high-purity population of human CD4+ T cells. Akadeum’s microbubble workflow is exceptionally gentle on cells, helping to maintain the health and physiology of immune cells during enrichment. The kit uses a negative selection method to remove unwanted cell populations from the sample, effectively enriching for the T cells of interest while maintaining cellular physiology. No magnet required. The undesired (non-CD4+ T) cells are labeled with biotinylated antibodies targeting CD8, CD11b, CD14, CD16, CD19, CD36, CD56, CD123, CD235a, and TCR-γδ. Streptavidin-coated microbubbles are mixed into the sample, binding to the labeled cells and floating them to the top of the sample container for removal. Capacity: 1 billion cells.